Anthrenus chikatunovi Holloway, 2020 is frequently confused with Anthrenus isabellinus Küster, 1848 (Coleoptera, Dermestidae, Megatominae). This has led to two issues, namely whether A. chikatunovi is a valid species, and if so, how to differentiate between the two species. Images and measurements of the aedeagi are presented that clearly show differences between the species, removing any doubt that A. chikatunovi is a different and valid species. How to identify A. chikatunovi from images alone, and how to differentiate it from A. isabellinus is discussed. Distributions of the two species in the Iberian Peninsula are presented, indicating that overlap is currently only known to occur in the north-east of Spain.